TABLE 3.
An exogenous NLS can functionally rescue carboxy-terminal deletions of Rep1p and Rep2p in plasmid maintenancea
| Rep source | Host | Test plasmid | SI | Cellular localization |
|---|---|---|---|---|
| Rep1p | ||||
| pGFP-Rep1p | [cir0] | pSTB2 | 62 | Nuclear |
| Δ25 | [cir0] | pSTB2 | <10 | Nonspecific |
| ΔC25-NLS | [cir0] | pSTB2 | 70 | Nuclear |
| NLS-ΔC25 | [cir0] | pSTB2 | 70 | Nuclear |
| Rep2p | ||||
| pGFP-Rep2p | [cir0] | pSTB1 | 65 | Nuclear |
| ΔC20 | [cir0] | pSTB1 | <10 | Nonspecific |
| ΔC20-NLS | [cir0] | pSTB1 | 72 | Nuclear |
| NLS-ΔC20 | [cir0] | pSTB1 | 72 | Nuclear |
a
The stability assays were carried out in a [cir0] host as described in Table 2. The NLS prefix indicates fusion of this sequence at the amino terminus, and the NLS suffix indicates fusion to the carboxy terminus. The cellular localization of the deletion protein with and without the NLS is indicated.