Figure S4.

Structure/function analysis of RIC-7. (A) Transcript expression level of ric-7, itr-1, and mig-13 in DA9 (threshold = 2) (Taylor et al., 2021). ric-7 is so lowly expressed that it’s not detected in DA9. (B) Schematic representations of the three splicing isoforms of ric-7. (C) Transcript expression level of the three splicing isoforms of ric-7 in different neurons. Note that ric-7b has a relatively higher expression than the other two isoforms. (D) Representative images showing the localization of 1–99 aa and 100–470 aa in the cell body expressed as transgenes driven by the promoter of Pmig-13 in different genotypes (see Materials and methods). (E) Representative images showing the localization of 1–99 aa and 100–470 aa in the dendrite expressed as transgenes driven by the promoter of Pmig-13 in ric-7 mutant animals. (F) Quantification of the percentage of mitochondria associated with EGFP signals. Number of mitochondria counted are indicated at the top of each bar. Error bars represent 95% confidence intervals. Total number of animals is seven for each condition. **P < 0.01 (Fisher’s exact test).