Figure S5.

Human Otulin or OtulinL, or the C-term domain of RIC-7 cannot rescue mitochondria localization in ric-7 mutant. (A) Protein remote homology detection for RIC-7b using HHPred (https://toolkit.tuebingen.mpg.de/tools/hhpred). Human proteome was selected as the search database. The top two hits were OtulinL and Otulin. Sequence alignment between RIC-7b and Otulin is shown in this figure. Note the high similarity of the secondary structures of the Query (Q1261352: RIC-7b) and Target (T 619C_A: Otulin). SS_Pred denote the PSI-PRED secondary structure prediction and “ss_dssp” is the actual secondary structure determined by the program DSSP. Quality of the column–column match: | very good, + good, · neutral, − bad, and = very bad. (B) Representative images showing mitochondria localization in ric-7 mutant animals expressing the human Otulin or OtulinL, or the C-term domain (471–709 aa) of RIC-7 driven by a pan-neuronal promoter from a single copy insertion.