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. Author manuscript; available in PMC: 2024 Mar 13.
Published in final edited form as: Bone. 2023 Nov 25;179:116983. doi: 10.1016/j.bone.2023.116983

Figure 2. PTH treatment for six days, with stress fracture loading on day three, resulted in increased macrophage infiltration into the callus site.

Figure 2.

(A) Experimental design with vehicle or PTH treatment started two days before loading the ulna for a stress fracture. Mice were euthanized 24 hours after the last treatment. (B,C) Sections stained with F4/80 antibody to quantify macrophage infiltration within the callus (outlined) with either vehicle or PTH treatment. (D) Quantification of F4/80 positive cell numbers per callus area. (E, F) Representative images (chosen by values closest to mean BV/TV) of stress fracture callus microCT renderings treated with either vehicle or PTH. (G, H) Callus bone volume per total volume (BV/TV) and callus bone volume analyzed by microCT are reported. Data was analyzed by T-tests using Prism software. *p<0.05, n=7–9/group