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Permeability of rHF nanoparticles across BBB model. (a) Intracellular trafficking of Cy5.5-labeled rHF nanoparticles in bEnd.3 cells, Scale bar = 20 μm. (b) Schematic illustration of the transwell assay for constructing the BBB model in vitro. (c) Internalization of penetrative rHF nanoparticles in the lower chamber of the system with PC12 cells after transporting across BBB for 24 h, Scale bar = 100 μm.
