FIG. 8.

Colocalization of Rb and Raf-1 in the nucleus. HSF8 cells were fixed after 60 min of serum stimulation and then immunostained with an anti-Raf-1 antibody (against residues 23 to 269) and an anti-Rb mouse monoclonal antibody. A fluorescein-conjugated anti-rabbit antibody or a rhodamine-conjugated anti-mouse antibody was used to visualize the staining. Arrows indicate the regions that were stained by both of the antibodies. Superimposition of the two images shows the regions of colocalization in yellow (bottom panel).