TABLE 3.
Concordance of qHTS KS, SPE–MS/MS DPRA-C, SPE–MS/MS DPRA-K, and IL-8 HTRF assays (based on the curve rank criteria) and OECD Toolbox automated workflow predictions for 288 chemicals. CD86/CD54 surface expression is not included for curve rank criteria because it was tested at a single concentration. The compounds detected as active and inactive assays were counted separately. The gray area is for concordance of compounds detected as active in assays. The blue area is for concordance of compounds detected as inactive in assays.
| Curve rank criteria | Number of chemicals detected as active in assays | ||||
|---|---|---|---|---|---|
| Method | qHTS KS (117) | SPE–MS/MS DPRA-C (128) | SPE–MS/MS DPRA-K (8) | IL-8 HTRF (52) | OECD TB*(174) |
| qHTS KS (171) | 60 | 1 | 24 | 80 | |
| SPE–MS/MS DPRA-C (149) | 97 | 8 | 28 | 88 | |
| SPE–MS/MS DPRA-K (269) | 158 | 149 | 1 | 6 | |
| IL-8 HTRF (236) | 143 | 128 | 221 | 33 | |
| OECD TB* (32) | 23 | 19 | 30 | 27 | |
| Number of chemicals detected as inactive in assays | |||||
*71 out of domain chemicals are not included.