Table 2.
Sequences and nucleotide positions of primer pairs for the detection of total NR1I2 and the three NR1I2 splice variants PXR.1, PXR.2, and PXR.3
| Name | Primer sequence | Nucleotide position# | Reference |
|---|---|---|---|
| PXR.1-forward | 5′-ACT TAC CAC CAA GCA GTC C-3′ | 185 to 203 | * |
| PXR.1-reverse | 5′-TCA AAG AGC ACA GAT CTT TCC-3′ | 830 to 850 | * |
| PXR.2-forward | 5′-AAG GAC AGC AGC ATG ACA G-3′ | 37 to 55 | * |
| PXR.2-reverse | 5′-CAA AGT CAG CAT GGT TCC AG-3′ | 189 to 208 | * |
| PXR.3-forward | 5′-TCA AGA ATT TCC GGG TCT CTC-3′ | 728 to 748 | Lamba et al. (2004) |
| PXR.3-reverse | 5′-CGA TGG GCA AGT CCC TGA AG-3′ | 900 to 919 | Lamba et al. (2004) |
| Total NR1I2-forward | 5′- CCC AGC CTG CTC ATA GGT TC- 3′ | 1367 to 1386 | Svecova et al. (2008) |
| Total NR1I2-reverse | 5′-CTG TGA TGC CGA ACA ACT CC-3′ | 1500 to 1519 | Svecova et al. (2008) |
#Primer positions were evaluated by the online tool available at https://www.bioinformatics.org/sms2/primer_map.html using the respective cDNA sequences available at https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
*Primers were designed for this study by means of the online tool available at https://eurofinsgenomics.eu/de/ecom/tools/pcr-primer-design/