FIG. 1.

(A) Location on the mtDNA of tRNA genes relative to the large (LSU)- and small (SSU)-subunit rRNA genes. tRNA genes are designated by the single-letter code for their cognate amino acid. Arrows indicate the direction of transcription of these genes. The lines labeled 1, 2, and 3 indicate the location and extent of hybridization probes used in Northern blots. (B) Northern blot of Physarum mitochondrial RNA (mtRNA). Mitochondrial RNA was separated by agarose-urea gel electrophoresis and transferred in situ to diazobenzyloxymethyl-paper. Equivalent strips were hybridized with probes 1, 2, and 3 (A). The detected RNAs are the mitochondrial large-subunit rRNA (lane 1), the mitochondrial small-subunit rRNA (lane 2), and tRNAs (lane 3).