Fig. 1. Characterization of dividing cells after targeted depletion of Scgb1a1⁺ cells.

a Schematic of Scgb1a1-CreER, Rosa26R-DTA, and CycB1-GFP transgenes in SRC mice. b IF staining of SRC mouse lungs with Cyp2f2 (white, club cells), GFP (green, dividing cells), and DAPI (blue, nuclei), before (day 0) and after 2 and 3 days of a single tamoxifen injection. GFP⁺ alveolar cells (arrow heads) and GFP⁺ spindle-like cells (arrows) can be observed on day 3. Aw: airway. Scale bar: 100 µm. Images are representative of five independent experiments with n = 3 animals analyzed per timepoint. c Scheme of cell sorting and scRNA-seq strategy. Created with BioRender.com. d–f UMAP embedding of scRNA-seq data from GFP⁺ cells sorted from lungs of SRC mice two days (n = 3 mice) and three days (n = 2 mice) after tamoxifen injection. d Cell cycle phase distribution. e Normalized expression of Epcam (epithelial cells) and Col1a1 (mesenchymal cells). f Cell type assignment. g Heatmap of the top ten upregulated genes across cell populations ranked by power (roc test). Scaling of expression was done after downsampling to 100 cells per cell type. DEGs mentioned in the text are in bold. h Percentage of dividing cell types in the distal lung at day 2 and 3 after tamoxifen injection.