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. 2024 Mar 12;9:66. doi: 10.1038/s41392-024-01781-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — KAL knockout improves hepatic steatosis, inflammatory infiltration and fibrosis of NAFLD rats. a The serum TG of rats fed with HFruD for 16 weeks. b, c mRNA levels of Serpina4, Oil red O staining and steatosis score of livers from the rats fed with HFruD for 16 weeks. d mRNA levels of Serpina4 in the livers from rats fed with MCD. e Oil red O staining and steatosis score of livers from the rats fed with MCD for 4 weeks. f Oil red O staining and steatosis score, H&E staining and NAS, Sirius staining and fibrosis scores of livers from the rats fed with MCD for 10 weeks. g The mRNA levels of Tnf, Acta2 and Col1a1 in the livers of rats fed with MCD for 10 weeks. h Immunoblot of TNFα, α-SMA, and Collagen I in the liver tissues from rats fed with MCD for 10 weeks, the black arrow represents the destination blots. Scale bar for Oil red O staining: 500 μm, for H&E staining and Sirius staining: 200 μm. Data are expressed as mean ± SD of no less than 4 rats per group. *p < 0.05, **p < 0.01, ***p < 0.001