Fig. 7.

KAL regulates KLF4 and PPARγ by activating GSK3β. a–c Protein levels of non-p-β-catenin, p-GSK3β, p-LRP6 (a), p-PKA (c), and the IP blot of KAL and LRP6 (b) in hepatocytes treated with Ad-KAL for 48 h. d Protein levels of p-LRP6, p-PKA, p-GSK3β in liver tissues of 16-month-old mice. e, f Representative immunoblot and quantification of protein levels, mRNA levels of ATGL and PPARγ in hepatocytes treated with Ad-KAL and LiCl (20 mM) for 48 h. g, h Protein levels and mRNA levels of CGI-58 in hepatocytes treated with LiCl (20 mM) for 48 h. i Protein level of CGI-58 in hepatocytes treated with LiCl (20 mM) and si-β-catenin for 48 h. j, k Representative immunoblot and quantification of protein levels, mRNA levels of CGI-58 and KLF4 in hepatocytes treated with Ad-KAL and LiCl (20 mM) for 48 h. l Representative immunoblot and quantification of KLF4 in nuclear extracts of hepatocytes treated with Ad-KAL and LiCl (20 mM) for 48 h. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001