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. 2024 Feb 13;25(3):1055–1074. doi: 10.1038/s44319-024-00092-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A, B) Immunofluorescence images (A) and quantification of the density of cilia (B) in LX-2 cells treated with TGF-β for 24 h (n = 6 independent experiments). To quantify the percentage of ciliated cells (B), >140 cells were analyzed for each experiment. Scale bar, 10 µm. (C) IFT88 mRNA expression was measured by quantitative RT-PCR after TGF-β treatment for 24 h in LX-2 cells (n = 3 independent experiments). (D–F) Immunoblotting (D) of IFT88 and α-SMA in LX-2 cells treated with TGF-β, and the levels of IFT88 (E) and α-SMA (F) were quantified by densitometry (n = 3 independent experiments). (G, H) The effect of TGF-β on the half-life of IFT88 in LX-2 cells treated with CHX (20 mg/mL) was examined by immunoblotting (G), and the protein half-life curves were obtained (H) (n = 3 independent experiments). (I, J) LX-2 cells were treated with TGF-β for 24 h and then treated with MG132 (5 mM) for 12 h. The levels of IFT88 and GAPDH were examined by immunoblotting (I), and the level of IFT88 was determined by densitometry (J) (n = 3 independent experiments). Data information: Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA with post hoc tests (E, F) or unpaired two-tailed Student’s t test (B, C, H, J). ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001. Related to Fig. 3. Source data are available online for this figure.