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. 2024 Feb 13;25(3):1055–1074. doi: 10.1038/s44319-024-00092-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A, B) Immunoblotting (A) and quantification (B) of the levels of IFT88 and α-SMA in HSCs isolated from CCl₄- or corn oil (vehicle)-treated Ift88^fl/fl and Ift88^fl/fl;Pdgfrb-CreER mice for 1 month (n = 4 mice). (C, D) Examination of AST (C) and ALT (D) activities in the serum of Ift88^fl/fl and Ift88^fl/f;Pdgfrb-CreER mice treated with CCl₄ or vehicle for 1 month (n = 4 mice). (E–G) Immunofluorescence images (E) and quantification of the levels of α-SMA (F) and vimentin (G) in the liver of Ift88^fl/fl and Ift88^fl/fl;Pdgfrb-CreER mice treated with CCl₄ or vehicle for 1 month (n = 6 mice). Nuclei were stained with DAPI (blue). Scale bar, 100 μm. (H, I) CCl₄-induced liver fibrosis in Ift88^fl/fl and Ift88^fl/fl;Pdgfrb-CreER mice was examined with Sirius red staining and H&E staining (H), and the percentage of collagen-positive areas was quantified (I) (n = 6 mice). Scale bars for Sirius red staining and H&E staining, 200 μm. Scale bar for liver, 1 cm. (J–N) The mRNA levels of IL-6 (J), IFNAR2 (K), TNF-α (L), COL1A1 (M), and α-SMA (N) in liver tissues were measured by quantitative RT-PCR (n = 4 mice). Data information: Data are presented as mean ± SD. Statistical significance was determined by two-way ANOVA with post hoc tests. ns not significant; **P < 0.01, ***P < 0.001. See also Figs. EV3 and EV4. Source data are available online for this figure.