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. 2024 Mar 12;10:132. doi: 10.1038/s41420-024-01903-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Pull-down assay experimental design. Biotinylated HGDILnc1 and antisense-HGDILnc1 RNA were incubated with HUVEC cell lysates. B Silver staining of HGDILnc1-associated proteins. Five HGDILnc1-associated bands (rectangular box) were excised and analyzed by mass spectrometry. C GO enrichment of HGDILnc1-associated proteins after RNA pull-down. D–F Lactate production (D), glucose uptake (E), and ATP production (F) in HGDILnc1-silenced HUVEC cells by colorimetric analysis. G Extracellular acid ratio (ECAR) in HGDILnc1-silenced HUVEC cells. OM oligomycin, 2-DG 2-deoxyglucose. H Top 15 HGDILnc1-associated cellular proteins according to the −10lgP among the HGDILnc1-RNA pull-down proteins. I Western blot of ENO1 from antisense HGDILnc1 and HGDILnc1 pull-down assays. J RNA immunoprecipitation with anti-ENO1 antibody and specific primers were used to detect HGDILnc1. β-actin was used as a negative control. K Binding capacity of ENO1 to HGDILnc1 following RNA immunoprecipitation using an antibody against ENO1 in HUVEC cell lysates after measurement of glucose deprivation, hypoxia, or glucose deprivation with hypoxia treatment by qRT-PCR. L Western blot of ENO1 in samples pulled down by full-length (FL) or truncated HGDILnc1 (F1: 1–500, F2: 501–900, F3: 901–1304). M Lactate production (left), glucose uptake (middle), and ATP production (right) were measured in HUVEC cells overexpressing the truncated HGDILnc1 F3 fragment by colorimetric analysis. N Extracellular acid ratio (ECAR) in cells after overexpression of the truncated HGDILnc1 F3 fragment in HUVEC cells. OM oligomycin, 2-DG 2-deoxyglucose. O Capillary tube formation for evaluating angiogenesis in HUVECs after overexpression of the truncated HGDILnc1 F3 fragment. P H&E-stained 10 µm paraffin sections (left) and CD31-labeled paraffin sections (right) of the Matrigel plugs after overexpression of truncated HGDILnc1 F3 fragment in an in vivo Matrigel implantation model.