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. 2024 Mar 12;10:132. doi: 10.1038/s41420-024-01903-w

Fig. 6. HGDILnc1 regulates the transcription of ALDOC through upregulation of H2BK16ac levels in the ALDOC promoter.

Fig. 6

A Left: schematic illustration for aerobic glycolysis-related enzyme genes. Right: Heatmap showing differentially expressed glycolysis-related enzyme genes between HGDILnc1-silenced cells and controls, by RNA-sequencing. Asterisk (*) indicates a significant difference in expression of the indicated gene between HGDILnc1-silenced cells and controls. B mRNA expression levels of potential differentially expressed aerobic glycolysis-related genes evaluated by qRT–PCR in HGDILnc1-silenced HUVECs (left) or HGDILnc1-overexpressing cells (right). C Western blot of potential differentially expressed aerobic glycolysis-related proteins in HGDILnc1-silenced HUVEC cells (left) or HGDILnc1-overexpressing cells (right). D Western blot of histone H2B from antisense HGDILnc1 and HGDILnc1 pull-down assays. E RNA immunoprecipitation experiments were performed using an anti-H2B antibody, and specific primers were used to detect HGDILnc1. β-actin was used as a negative control. F Binding capacity of candidate H2B acetylation to the ALDOC promoter measured by ChIP-qPCR after knocking down HGDILnc1. An IgG antibody was used as a negative control. G Western blot of histone H2BK16ac from antisense HGDILnc1 and HGDILnc1 pull-down assays. H RNA immunoprecipitation experiments were performed using an anti-H2BK16ac antibody, and specific primers were used to detect HGDILnc1. β-actin was used as a negative control. I Lactate production (left), glucose uptake (middle) and ATP production (right) were measured in HGDILnc1-silenced HUVEC cells with transfection of ENO1 or ALDOC or co-transfection of ENO1 and ALDOC, shown by colorimetric analysis. J Extracellular acid ratio (ECAR) in HGDILnc1-silenced HUVEC cells with transfection of ENO1 or ALDOC or co-transfection of ENO1 and ALDOC. OM oligomycin, 2-DG 2-deoxyglucose. K Capillary tube formation for evaluating angiogenesis in HGDILnc1-silenced HUVEC cells with transfection of ENO1 or ALDOC or co-transfection of ENO1 and ALDOC. L Representative images of H&E-stained 10 µm paraffin sections (left) and CD31-labeled paraffin sections (right) of the Matrigel plugs after HGDILnc1 silencing with overexpression of ENO1 or ALDOC or co-overexpression of ENO1 and ALDOC in an in vivo Matrigel implantation model.