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. 2024 Feb 8;25(3):1022–1054. doi: 10.1038/s44319-024-00075-z

Figure 2. TGFβ induces LINC00313 through TβRI/Smad-dependent pathways.

Figure 2

(A) LINC00313 expression in HuCCT1 and Huh28 cells treated with LY2157299 or DMSO, and stimulated with TGFβ1 or BSA/HCl for 16 h. (B) LINC00313 and SERPINE1 expression in HuCCT1 and Huh28 treated with SIS3 or DMSO and stimulated with TGFβ1 or BSA/HCl for 16 h. (C) LINC00313 expression in HuCCT1 transiently transfected with siRNA targeting SMAD2, SMAD3, or SMAD4, alone or in combination, with or without TGFβ1. (D) LINC00313 expression in Huh28 transiently transfected with siRNA targeting SMAD2, SMAD3, or SMAD4, alone or in combination, with or without TGFβ1. (E) Snapshot of the UCSC genome browser showing predicted SMAD-binding regions in LINC00313 gene locus, using the JASPAR CORE 2022 collection. Active histone mark (H3K27ac) is also shown around LINC00313 TSS. Data information: Data are presented as mean ± SD (n = 3 biological replicates). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (Student’s t-test), n.s.: not significant. In panel (A) the experiment in HuCCT1 cells was performed twice (n = 2 biological replicates) and data are presented as single data points. Source data are available online for this figure.