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. 2023 Dec 25;11(10):2304761. doi: 10.1002/advs.202304761

Figure 3.

Figure 3

Pro‐differentiation capacity and bioactivity of LMGDNPs against lactate. A) CCK8 assay detecting the viability of BMSCs cultured in pellets or on microspheres. Data are presented as the mean ± SD, n = 3, ns, no significance, *p < 0.05 between DNP and GelMA group; #p < 0.05 between LMGDNP and DNP group. B) Evaluation of the viability of BMSCs cultured on microspheres with or without lactate for 24 h. C) Fluorescence images of the live/dead assays of BMSCs cultured on microspheres with or without lactate for 24 h. Scale bar: 100 µm. D) Representative immunocytochemistry images showing the expression of NPCs markers (Krt19, CD24, Col2, and Acan) in BMSCs cultured on microspheres for 21 days. Genipin was visualized using orange fluorescence. Scale bar: 100 µm. E) Relative mRNA expression of Krt19, CD24, Col2, and Acan in BMSCs cultured on microspheres for 14 and 21 days. Data are presented as the mean ± SD, n = 3, ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001 between groups. DNP, decellularized nucleus pulposus matrix hydrogel‐based microspheres. GDNP, glucose‐rich nucleus pulposus matrix hydrogel‐based microspheres. LMGDNP, LOX‐MnO2 nanozyme‐loaded glucose‐rich nucleus pulposus matrix hydrogel‐based microspheres.