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. 2023 Dec 25;11(10):2305563. doi: 10.1002/advs.202305563

Figure 1.

Figure 1

VDR activation alleviated the injury of HK‐2 cells cultured in HG medium. Cell viability was quantified after the cells were cultured in different doses of PAR or Fer‐1 with 30 mm glucose medium for 48 h (A,B). The expression of Kim‐1 and NGAL was examined by western blotting analysis, followed by densitometric analysis of the blots. β‐actin served as a loading control (C). Representative micrographs of immunofluorescence staining for ROS (green) in different groups. Quantitative statistical analysis of the fluorescence intensity of ROS per fixed field was performed. Scale bar = 20 µm (D). Each bar represents the mean ± SD of the data derived from three independent experiments (n = 3). ** p < 0.01 versus NG group; ## p < 0.01 versus HG group; && p < 0.01 versus HG group.