Figure 7.

Nrf2 deficiency eliminated the antiferroptotic effect of VDR. Trig was pretreated to HG‐cultured HK‐2 cells for 30 min. Western blotting and related densitometric analysis were applied to detect the expression levels of VDR, Nrf2, and HO‐1. β‐actin served as a loading control(A). HK‐2 cells were transfected with Nrf2‐specific siRNA or negative control siRNA under high glucose conditions. Western blotting was used to detect the expression levels of Nrf2, HO‐1, SLC7A11, GPX4, TFR‐1, FTH‐1, and FSP1 with or without PAR intervention, followed by densitometric analysis of the blots. β‐actin served as a loading control (B). Quantitative analysis of iron (C), MDA (D), and GSH (E) levels in each cell group. Each bar represents the mean ± SD of the data derived from three independent experiments (n = 3). ^** p < 0.01 versus HG group; ^{^^} p < 0.01 versus HG+PAR group; ^† p < 0.05, ^†† p < 0.01 versus HG group and HG+si‐Ctrl group; ns indicates no significance.