TABLE 1.
Inhibitor profile of eIF4G cleavage activity with in vitro cell lysate assays
| Protease inhibitora | Inhibitionb |
|---|---|
| Ac-DEVD-CHO (10 nM) | ++ |
| Ac-YVAD-CMK (1 μM) | ++ |
| Antipain (100 μM) | − |
| Aprotinin (300 mM) | − |
| Elastatinal (100 μM) | +/− |
| E-64 (100 μM) | +/− |
| Iodoacetamide (100 μM) | ++ |
| Leupeptin (100 μM) | − |
| Pepstatin (10 μM) | − |
| 1,10-Phenanthroline (10 mM) | ++ |
| PMSF (1 mM) | − |
| TLCK (100 μM) | ++ |
| TPCK (100 μM) | ++ |
| Trypsin inhibitor (soybean) (100 μM) | + |
a
The inhibitor concentrations listed represent the lowest values tested in multiple assays.
b
−, no inhibition; +/−, 25% inhibition or less; +, 50% inhibition; ++, >50% inhibition of eIF4G cleavage in vitro during incubation at 37°C for 18 h.