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. 2024 Feb 28;13(5):677. doi: 10.3390/plants13050677

Figure 4.

Figure 4

RT-PCR analysis of key genes involved in chilling injury in the “Big Top” cv. budded on different Prunus rootstocks. Data were normalized using “GF 677” expression at harvest as control. Means showed the average for 2020 and 2021 seasons at harvest and after 28 days of cold storage at 5 °C plus 2 days of shelf life (28 + 2 d). Bars represent the standard errors of the means. Means with different letters are significantly different (p ˂ 0.05) according to Duncan’s test. (a) Phenylalanine Ammonia Lyase 1 (PAL1). (b) Peroxidase 2 (POD2). (c) Polyphenol Oxidase 4 (PPO4). (d) Catalase 1 (CAT1). (e) Pectin Methylesterase 1 (PME1). (f) Polygalacturonase 2 (PG2). (g) Expasin 3 (EXP3). (h) Chalcone Synthase 2 (CHI2). (i) Leucoanthocyanidin Dioxygenase (LDOX). (j) Heat map. The different colors represent the increase or decline in the expression at 28 + 2 d in comparison with the harvest.