Table 2.
Statistical significance of rootstock, treatment, year and their interaction on “Big Top” for gene-relative expressions.
| Gene | Variance Analysis | Variability (%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T | R | Y | T*R | T*Y | Y*R | T*R*Y | T | R | Y | T*R | T*Y | Y*R | T*R*Y | |
| Phenylalanine Ammonia Lyase 1 (PAL1) | ns | * | ns | ns | ns | ns | ns | 5.2 | 16.9 | 9.8 | 7.7 | 1.1 | 25.8 | 12.8 |
| Polyphenol Oxidase 4 (PPO4) | * | ns | ns | ns | ns | ** | ns | 0.6 | 35.3 | 4.1 | 1.3 | 0.5 | 34.4 | 1.4 |
| Peroxidase 2 (POD2) | ns | ** | ns | ns | ns | ns | ns | 1.1 | 18.3 | 6.8 | 17.3 | 10.7 | 25.0 | 11.8 |
| Catalase 1 (CAT1) | * | *** | *** | ns | ns | *** | ns | 2.6 | 45.9 | 17.1 | 7.5 | 1.1 | 20.1 | 7.5 |
| Pectin Methylesterase 1 (PME1) | ns | ** | ns | ns | ns | ns | ns | 2.1 | 39.9 | 2.1 | 13.7 | 1.0 | 18.6 | 4.5 |
| Polygalacturonase 2 (PG2) | *** | ** | ** | ns | *** | ns | ns | 69.8 | 6.6 | 3.5 | 2.4 | 5.4 | 4.3 | 2.7 |
| Expansin 3 (EXP3) | * | ns | *** | * | ns | * | * | 0.2 | 12.9 | 16.5 | 22.4 | 2.9 | 19.4 | 21.4 |
| Chalcone Synthase 2 (CHI2) | *** | ns | *** | ns | ns | ns | ns | 25.3 | 3.4 | 23.5 | 3.3 | 3.9 | 15.4 | 5.1 |
| Leucoanthocyanidin Dioxygenase (LDOX) | ns | ns | ns | ns | ns | ns | ns | 3.1 | 19.2 | 4.2 | 10.6 | 1.2 | 28.2 | 15.3 |
Data were evaluated by three-way variance analysis (ANOVA) on raw data from 2020 and 2021 seasons at harvest and after 28 days plus 2 days of shelf life. Significance: * indicates p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and ns p > 0.05. T, Treatment (harvest and 28 days at 5 °C + 2 days of shelf life). R, Rootstock. Y, Year.