Figure 5.

Effect of GNL on Ox-LDL-induced ROS production in HUVECs. (A) The HUVECs were then pretreated with GNL (0, 50 and 100 µM, for 2 h), followed by Ox-LDL (100 µg/mL) for 24 h. We measured the intracellular ROS levels using DCF fluorescence. (B) LPO, SOD and CAT. Based on the manufacturer’s instructions, we used ELISA kits. There are three replicates of each value, and * represents p < 0.05; thus, there is a significant difference when compared to the control group. The # represents p < 0.05; thus, there are significant differences between the Ox-LDL alone and GNL with Ox-LDL treatment groups.