Table 1. Details of the PCR Primers Used.
name | target | sequence | amplicon size | specificity |
---|---|---|---|---|
universal bacterial primers32 | 16s rDNA gene | forward primer 27F (5′-AGA GTT TGA TCC TGG CTC AG-3′) and reverse primer 1492R (5′-TAC CTT GTT ACG ACT T-3′) | ∼1400 bp | most common bacterial species, not fungi |
type III primers33 | SCC MecIII gene | (5′-CCA TAT TGT GTA CGA TGC G-3′) type III-R (5′-CCT TAG TTG TCG TAA CAG ATC G-3′) | 280 bp | methicillin-resistant Staphylococcus aureus |
HSV-2 primers34 | DNA polymerase gene | (5′ GAC AGC GAA TTC GAG ATG CTG 3′) reverse (5′ ATG TTG TAC CCG GTC ACG AAC T 3′) | 80 bp | Herpes Simplex Virus Type 2 |