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. 2024 Feb 16;25(3):1629–1636. doi: 10.1021/acs.biomac.3c01187

Table 1. Details of the PCR Primers Used.

name target sequence amplicon size specificity
universal bacterial primers32 16s rDNA gene forward primer 27F (5′-AGA GTT TGA TCC TGG CTC AG-3′) and reverse primer 1492R (5′-TAC CTT GTT ACG ACT T-3′) ∼1400 bp most common bacterial species, not fungi
type III primers33 SCC MecIII gene (5′-CCA TAT TGT GTA CGA TGC G-3′) type III-R (5′-CCT TAG TTG TCG TAA CAG ATC G-3′) 280 bp methicillin-resistant Staphylococcus aureus
HSV-2 primers34 DNA polymerase gene (5′ GAC AGC GAA TTC GAG ATG CTG 3′) reverse (5′ ATG TTG TAC CCG GTC ACG AAC T 3′) 80 bp Herpes Simplex Virus Type 2