FIG. 1.

(A) Genomic organization of expression constructs. Amino acids in capital letters are part of the PV genome; lowercase amino acids mark exogenous sequences, with boxes indicating the coding sequences of the inserted genes. The PV ORF initiating AUG is in its original context; the first 3 aa of VP4 (GAQ) are duplicated. The differences in pmogfp and pmoHgag are a more extensive multiple cloning site, six glycine residues upstream the cleavage site instead of four, and inclusion of the original P5 residue (glutamate) into the 3C^pro/3CD^pro recognition site. (B) Schematic of the oligonucleotides used as RT-PCR primers and resulting fragment lengths.