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Determination of the ratio of moHgag deletion variants during progressive passaging. Viruses were plaque purified from initial RNA transfection (A), first passages (A and B), and sixth passages (C). Viral RNAs were subjected to RT-PCR with flanking primers 7215 and 6509. M, DNA molecular weight marker VI (Boehringer Mannheim); T, RT-PCR of pmoHgag transcript RNA; wt, RT-PCR of PV1(M) virion RNA. Products were run on a 1.2% agarose gel (ethidium bromide stained).
