FIG. 2.

Thirty percent sucrose cushion analysis of HPV-11 VLP disassembly. HPV-11 preparations were treated at 4°C as described in the text, and samples were taken at the top (T) or bottom (B) of the sucrose cushion prior to gel electrophoresis. Group 1, untreated, purified HPV-11 VLP starting material in PBS; group 2, VLPs incubated with 5% βME for 16 h; group 3, VLPs incubated with 5% βME for 1 h; group 4, VLPs incubated with 2% βME for 16 h; group 5, VLPs incubated with 0.5% βME for 16 h; group 6, VLPs incubated with 10 mM DTT–5 mM EDTA for 16 h.