FIG. 3.

Five to 20% linear sucrose gradient analysis of disassembled HPV-11 VLPs. VLPs in PBS were incubated with 5% βME (a) or 200 mM NaHCO₃ (pH 9.6) (b) for 16 h at 4°C and then centrifuged on a 5 to 20% linear sucrose gradient as described in the text. The gradient was collected in 25 fractions (0.5 ml), and the pellet (P) was resuspended in 0.5 ml of PBS. Shown is an immunoblot demonstrating positions of the L1 protein across the gradient. Also indicated are the peak positions at which sedimentation standards migrated when run on separate gradients.