TABLE 2.

Effects of chelators and buffers on disassembly of HPV-11 L1 VLPs

Disassembly condition	Distribution (%)a
	Top	Bottom
Starting material	4 ± 2	96 ± 2
200 mM EDTA, pH 7.4	4 ± 3	96 ± 3
200 mM EDTA, 10 mM DTT	10 ± 6	90 ± 6
200 mM EGTA, pH 7.4	13 ± 11	87 ± 11
200 mM EGTA, 10 mM DTT	11 ± 6	89 ± 6
200 mM NaHCO3, pH 9.6	81 ± 2	19 ± 2
200 mM NaHCO3, 10 mM DTT	74 ± 11	26 ± 11
200 mM glycine, pH 9.6	11 ± 1	89 ± 1
200 mM glycine, 10 mM DTT	41 ± 12	59 ± 11

^aVLPs (0.5 to 1.0 mg of protein per ml) were treated as indicated for 16 h at 4°C, and the distribution of L1 across a 30% sucrose cushion was determined as described in Materials and Methods. Shown are the averages of duplicate determinations ± variation.