FIG. 1.

Northern blot analysis of VZV-infected BSC-1 RNA. (A) Total RNA (20 μg) extracted from control BSC-1 cells (lanes C) and VZV-infected BSC-1 cells (lanes V) along with RNA standards (lane M; Gibco-BRL) was resolved in 1% agarose gels containing 0.5 mM methylmercury(II) hydroxide and stained with 0.5 μg of ethidium bromide per ml in 0.5 M ammonium acetate. (B and C) RNA was transferred to a nylon-based membrane and probed for VZV gene 21 transcripts (B) or β-actin transcripts (C). VZV gene 21 transcripts are visible as a discrete 3.1-kb band in VZV-infected cell RNA. Both control and VZV-infected cell RNA contain discrete 1.8-kb β-actin transcripts.