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. 1998 Jan;72(1):42–47. doi: 10.1128/jvi.72.1.42-47.1998

TABLE 1.

Oligonucleotide primers

Primer Sequence (5′ to 3′)a 5′ startb 3′ endb Use
21pe1 CTTCTTGTACTTTCAAGTTAC 30808 30828 Primer extension
21pe2 GTTTTCTCTGGTGACCATGG 30853 30862 Primer extension
21pe3 GGAATTTTCTGGGTAGATCG 30897 30916 Primer extension
21-mlu GCGACGCGTAGCTGAGGGGTTAAATTCACA 30475 30497 5′-end p21, p21Δ
21-mluA GCGACGCGTGGTAGGAGGAGCC 30585 30597 5′-end p21A
21-mluB GCGACGCGTGGTTCTTCAACTTACCGTG 30626 30644 5′-end p21B
21-mluC GCGACGCGTGCGTTTTTATTGATGTTAC 30663 30682 5′-end p21C
21-mluZ GCGACGCGTCGGGGTCACGTCCAGCCTGTG 29979 30000 5′-end p21Z
21-kpn GCGGGTACCGGTATATTCTACGCTGACTTAAC 30758 30736 3′-end p21, p21A, p21B, p21C
21-kpnA GCGGGTACCGGCTCCTCCTACC 30597 30585 3′-end p21Δ
KpnI-MluI-p1 TACGCGTGGAATTCCGGTACCG NAc NA Modify pCAT3basic
KpnI-MluI-p2 CGCGCGGTACCGGAATTCCACGCGTAGTAC NA NA Modify pCAT3basic
4-HindIII CAAAATATCTGACAA(GC)TTGCGTGTTTGCAG 4147 4175 Introduce HindIII site 5′ of ORF 4
4-SpeI CAAATTAGTATGTTTTGAC(TAGT)AGCATGAAAAAGG 2739 2771 Introduce SpeI site 3′ of ORF 4
10-EcoRI CTTATTTAAACTAAAGA(A)TT(C)TTACTCTATAAG 12128 12158 Introduce EcoRI site 5′ of ORF 10
10-XbaI CGCGTTAAACGTC(TAG)ATTGGGGTAGAG 13385 13409 Introduce XbaI site 3′ of ORF 10
61-HindIII GAATACAGCCAA(G)CTTGTTACCATGG 104505 104482 Introduce HindIII site 5′ of ORF 61
61-SpeI GAAGTCCTAGTT(AC)T(A)GTTGGGAGGGGG 103091 103067 Introduce SpeI site 3′ of ORF 61
62-EcoRI GGGTACGTCTA(G)AATTCACCCCAG 109160 109138 Introduce EcoRI site 5′ of ORF 62d
63-HindIII GGTGCAAAACATGTCC(AAGC)TTGGGGCCGTAGTA 110592 110563 Introduce HindIII site 5′ of ORF 63
63-SpeI TGTATTTATTTATAA(CT)AG(T)ACTACACGCCATGGG 111435 111404 Introduce SpeI site 3′ of ORF 63
EcoRI-HindIII-p1 AATTCCTGGA NA NA Link EcoRI site to HindIII site in pCIneo
EcoRI-HindIII-p2 AGCTTCCAGG NA NA Link EcoRI site to HindIII site in pCIneo
a

MluI sites are indicated by a single underline. KpnI sites are indicated by a double underline. Parentheses enclose nucleotides added to introduce the desired restriction endonuclease. 

b

Nucleotide position of the oligonucleotide on the VZV genome. 

c

NA, not available. 

d

The 3′ cloning site (XbaI) was supplied by the pAlter-1 vector.