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qPCR validation confirmed modulation of miR-5099, miR-551b-3p and miR-146a-3p in the mouse kidney upon HFD feeding. (A–C) miRNAs levels were determined by real-time qPCR and expressed as fold change over STD (value = 1.0) after normalizing for both miR-30a-3p and miR-30b-5p, as endogenous controls. Data are presented as mean ± SEM (n = 7–9 mice/group).
