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. 2024 Feb 28;16(5):691. doi: 10.3390/nu16050691

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of NFκB, PI3K-Akt and MAPK pathway activation in the mouse kidney upon HFD feeding. Whole kidney lysates underwent protein analysis and were immunoblotted with antibodies against pAkt, pERK1/2, p-p38, Iκ-Bα, total ERK1/2 and GAPDH. (A) Representative Western blots. (B) Quantitative densitometric analysis. Data were normalized to GAPDH and presented as mean ± SEM (n = 6–7 mice/group) (fold change over STD). (C) mRNAs levels for NFκB p50 were determined by real-time qPCR and expressed as fold change over STD (value = 1.0) after normalizing for TBP. Data are presented as mean ± SEM (n = 8–9 mice/group).