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. 2023 Nov 4;14(3):1345–1361. doi: 10.1016/j.apsb.2023.11.005

Figure 2.

Figure 2

Photoimmune activation and downregulation of PD-L1 by PM@RM-T7 in vitro. (A) Representative CLSM images of cellular uptake of PC@RM-T7 and relevant controls by 4T1 cells after 6 h incubation. Relative cell viability of 4T1 cells treated with PM@RM-T7 and the relevant controls (scale bar = 100 μm) (B) under dark and (C) laser irradiation. (D) Representative flow cytometry plots of 4T1 cells after different treatments. (E) CRT exposure and (F) CLSM images of 4T1 cells with IF staining of CRT (scale bar = 50 μm). Expression of PD-L1 in the 4T1 cells detected by (G) western blotting and (H) IF staining after treatment with PM@RM-T7 and relevant controls with or without laser irradiation (scale bar = 50 μm). Data are presented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗∗P < 0.0001 vs. indicated.