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. 2023 Nov 4;14(3):1345–1361. doi: 10.1016/j.apsb.2023.11.005

Figure 3.

Figure 3

Selective recognition and reprogramming ability of PR@RM-M2 on M2 type RAW264.7 cells. (A) Representative CLSM images of M2 type RAW264.7 cells incubated with PC@RM, PC@RM-M2, or PC@RM-T7 for 6 h (scale bar = 100 μm). (B) Representative flow cytometry plots of M1 type RAW264.7 cells (CD86high) and M2 type RAW264.7 cells (CD206high) gating on F4/80+ after different treatments and (C) the corresponding statistical analysis. (D) TNF-α, IL-6, IFN-γ, and IL-10 secretion by M2 type RAW264.7 cells after different treatments. (E) CLSM images of IF staining of CD86 (green) and CD206 (red) on the surfaces of M2 type RAW264.7 cells after various treatments (scale bar = 50 μm; zoom in: scale bar = 20 μm). (F) Representative CLSM images of the phagocytosis of 4T1 cells (labeled by Hoechst) by M2 type RAW264.7 cells (labeled by DiO) after treatment of R848, PR@RM, and PR@RM-M2 (scale bar = 100 μm; zoom in: scale bar = 20 μm). Data are presented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗∗P < 0.0001 vs. indicated. n.s., not significant.