Assessment of synergistic photoimmunotherapeutic effect against 4T1 primary tumors. (A) Therapeutic schedule for 4T1 bilateral tumor-bearing mice. (B) The volumes, (C) weight, and (D) photographs of primary tumors after various treatments. Microscopic images of primary tumor sections stained with (E) HE (scale bar = 200 μm), TUNEL (scale bar = 50 μm), and immunofluorescence staining of (F) CRT, HSP 70, and (G) PD-L1 (scale bar = 100 μm). Data are presented as mean ± SD (n = 3). ∗∗P < 0.01 and ∗∗∗∗P < 0.0001 vs. indicated. n.s., not significant.