Disease symptoms of ArLV1-IL-Bh. (a,b) Disease progression symptoms on B. perviridis plants infected by ArLV1-IL-Bh. (a) Stripes of mild necrotic symptoms. (b) A ringspot phenotype. (c) Disease symptoms on N. benthamiana plants showing mosaic and leaf distortion. (d) RT-PCR detecting RNA1 and RNA2 of ArLV1-IL-Bh using primer pairs F10-R8 and F15-R13, respectively (Table 1) in B. hirta and Erucaria hispanica plants grown in the wild adjacent to domesticated and wild bee populations. 1–3, 5, 6, geographic collection regions of B. hirta: 1, Luzit; 2, Galon; 3, Lachish; 5, Tarum; 6, Agur; 4, Lachish- Erucaria hispanica plants. (e) RT-PCR detecting RNA1 and RNA2 of ArLV1-IL-Bh in bee samples. M, molecular size marker; Act, Actin; ntc, non-template control.