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. 2024 Feb 28;13(5):671. doi: 10.3390/plants13050671

Table 4.

BLAST analysis of HTS de novo assembled contigs derived from managed and wild bees’ pooled samples.

Honey bee (Apis mellifera)
Contig RNA Position (nt) Contig Size (bp) * Mismatch * INDELs % ArLV1-IL-Bh aa % ArLV1 aa % Zymoseptoria Comovirus A aa % AmCV aa
2688 1 124–492 368 7 0 99 82 82 98
3388 1 1327–1647 320 3 0 100 89 89 98
2241 1 1645–4430 2785 19 0 99 90 90 97
39_1 1 4362–5907 1545 26 0 99 83 83 94
39_3 2 1–3378 3378 56 4 99.8 79.4 79.9 92.19
Mining bee (Andrena)
3396 1 2715–3376 661 6 0 99.5 90.4 90.4 97.2
2094 1 3361–3624 263 2 0 100 89.7 89.7 98.8
3946 1 3642–4061 419 4 0 100 87.1 87.1 96.4
67_2 1 4164–5892 1728 30 1 99.6 85.2 85.2 94.6
2253 2 182–744 562 5 0 99 67 67 86
1218 2 959–1199 240 4 0 79 76 76 79
4073 2 1184–1501 317 6 0 99 66 68 90
67_1 2 1563–3443 1880 25 3 99 82 82 93
Long-horned bee (Eucera)
4418 1 4559–4820 261 7 0 99 92 92 98
2837 1 5051–5784 733 9 0 99 78 78 92
3346 2 498–744 246 2 0 99 92 93 95
2617 2 1057–1328 271 4 0 100 90 91 98
2924_1 2 1429–1634 205 5 0 97 53 77 90
2924_0 2 1612–2155 543 14 0 74 68 69 71
1924 2 2313–2926 613 9 0 97 79 79 95
4230 2 2912–3317 405 6 2 100 66 66 84
Masked bee (Hylaeus)
7428 1 3531–3924 393 6 0 85 73 73 80
9174 1 4032–4347 315 4 0 99 83 83 94
12,606 1 4704–4973 269 10 0 99 96 96 96
5150 1 5054–5597 543 7 0 100 90 90 99
12,033 2 822–1117 295 13 0 99 98 98 99
12,764 2 1548–1818 270 8 0 98 87 88 91
1842 2 1970–2208 238 2 0 100 86 86 94
4413 2 2193–3254 1061 23 1 98 78 89 93

* Mismatch and INDELs were determined on ArLV1-IL-Bh genome reference. ArLV1-IL-Bh, (OR840696, OR840697); ArLV1, (MH899120, MH899121); Zymoseptoria comovirus A (MK231051, MK231039); AmCV (OP972917-20); aa, denoted for amino acids.