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. 2023 Oct 28;14(3):1317–1328. doi: 10.1016/j.apsb.2023.10.018

Table 1.

Comparison of binding performances of the previously reported affinity materials.

Material Ligand Ligand density Target protein Binding capacity Ref.
Sepharose beads AviPure ligand 429 nmol/g IgG 53 mg/mL 38
Agarose-OPS adsorbent 31.93 mg/mL IgG 24.2 mg/mL 23
Porous membranes containing immobilized peptide KGSGSGSQLGPYELWELSH (KH19) 5.1 ± 1.3 mg/mL Trastuzumab 1.3 ± 0.3 mg/mL 37
Peptide immobilized monolith HHHHHHGSGSGSQLGPYELWELSH (HH24) 11 mg/mL Trastuzumab 16.4 mg/g 13
Peptide immobilized monolith Fmoc-HWEGWV 155 μmol/mL IgG 101.8 mg/mL 39
Fe3O4@SiO2 magnetic nanoparticles Protein A 203 mg/g anti-EGFR mAb 112.3 mg/g 24
Peptide immobilized monolith Histidine-tagged cyclic peptide 13.8 mg/mL IgG 119.3 mg/g 11
His-MWNTs l-Histidine 210 μmol/g IgG 267.8 mg/g 40
Porous membranes containing immobilized peptide KGSGSGSWPRWLEN (KN14) / Rituximab 16.5 mg/mL 19
Fe3O4@NiFe LDH@HN19
Fe3O4@NiFe LDH@HE24
HN19
HE24
2467 mg/g
2382 mg/g
Rituximab
Rituximab
1375 mg/g
1194 mg/g
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