Table 1:
Top 5 BLASTp hits from UniProt.
| Proteins | UniProt KB Protein Database | ||||
|---|---|---|---|---|---|
| Core Proteins | UniProt ID | Species | Protein description | E-value | BLAST score |
| PERIODA (PERA) | |||||
| LpPERA | H7C8F3 | Apteronemobius asahinai | PERIOD, isoform 1 | 1e-121 | 1,058 |
| H7C8F4 | Apteronemobius asahinai | PERIOD, isoform 2 | 2e-121 | 1,054 | |
| B7PDL3 | Ixodes scapularis | Period circadian protein, putative | 4e-118 | 1,013 | |
| Q8MMG2 | Blattella germanica | Circadian clock protein PERIOD | 1e-114 | 1,007 | |
| L0CAI9 | Rhyparobia maderae | Period | 2e-114 | 1,009 | |
| PERIODB (PERB) | |||||
| LpPERB | B7PDL3 | Ixodes scapularis | Period circadian protein, putative | 6e-119 | 1,020 |
| Q8MMG2 | Blattella germanica | Circadian clock protein PERIOD | 8e-118 | 1,031 | |
| A1EA98 | Blattella bisignata | Circadian clock protein period | 2e-115 | 1,008 | |
| H7C8F3 | Apteronemobius asahinai | PERIOD, isoform 1 | 2e-114 | 1,008 | |
| H7C8F4 | Apteronemobius asahinai | PERIOD, isoform 2 | 4e-114 | 1,004 | |
| PERIODC (PERC) | |||||
| LpPERc | H7C8F3 | Apteronemobius asahinai | PERIOD, isoform 1 | 4e-133 | 1,142 |
| H7C8F4 | Apteronemobius asahinai | PERIOD, isoform 2 | 3e-132 | 1,134 | |
| B7PDL3 | Ixodes scapularis | Period circadian protein, putative | 2e-129 | 1,095 | |
| L0CAI9 | Rhyparobia maderae | Period | 9e-129 | 1,115 | |
| A1EA98 | Blattella bisignata | Circadian clock protein period | 5e-124 | 1,071 | |
| TIMELESS (TIM) | |||||
| LpTIM | J9Y3V3 | Clunio marinus | Timeless | 2e-180 | 1,464 |
| G9M9V8 | Thermobia domestica | TIMELESS | 4e-178 | 1,458 | |
| H2D5T9 | Aedes albopictus | Timeless | 6e-165 | 1,370 | |
| E0D5C1 | Gryllus bimaculatus | TIMELESS | 5e-164 | 1,359 | |
| Q05E95 | Aedes aegypti | TIMELESS | 2e-161 | 1,349 | |
| CLOCK (CLK) | |||||
| LpCLK | E2C765 | Harpegnathos saltator | Circadian locomoter output cycles protein kaput | 4e-164 | 1,276 |
| H9KJ84 | Apis mellifera | Uncharacterized protein | 3e-161 | 1,264 | |
| E2AY34 | Camponotus floridanus | Circadian locomoter output cycles protein kaput | 3e-159 | 1,251 | |
| F4WUR0 | Acromyrmex echinatior | Circadian locomoter output cycles protein kaput | 1e-156 | 1,233 | |
| H9HL46 | Atta cephalotes | Uncharacterized protein | 4e-156 | 1,223 | |
| CYCLE 1 (CYC1) | |||||
| LpCYC1 | E0D6T3 | Thermobia domestica | CYCLE | <1e180 | 1,786 |
| A0MH07 | Lutzomyia longipalpis | Cycle | <1e180 | 1,622 | |
| E2A3F0 | Camponotus floridanus | Aryl hydrocarbon receptor nuclear translocator | <1e180 | 1,643 | |
| A9XCF1 | Tribolium castaneum | CYCLE | <1e180 | 1,621 | |
| E2B7K2 | Harpegnathos saltator | Aryl hydrocarbon receptor nuclear translocator | <1e180 | 1,620 | |
| CYCLE 2 (CYC2) | |||||
| LpCYC2 | E0D6T3 | Thermobia domestica | CYCLE | <1e180 | 1,674 |
| E2A3F0 | Camponotus floridanus | Aryl hydrocarbon receptor nuclear translocator | <1e180 | 1,606 | |
| H9K2D4 | Apis mellifera | CYC | <1e180 | 1,562 | |
| A0MH07 | Lutzomyia longipalpis | Cycle | <1e180 | 1,555 | |
| A9XCF1 | Tribolium castaneum | CYCLE | <1e180 | 1,560 | |
| CRYPTOCHROME 2 (npCRY2) | |||||
| LpnpCRY2 | E2BGC2 | Harpegnathos saltator | Cryptochrome-1 | <1e180 | 2,213 |
| L7YAB3 | Solenopsis invicta | Cryptochrome | <1e180 | 2,210 | |
| A8QSC1 | Bombus impatiens | Cryptochrome 2 protein | <1e180 | 2,202 | |
| L0C8K6 | Rhyparobia maderae | Cryptochrome 2 | <1e180 | 2,199 | |
| F4WVC9 | Acromyrmex echinatior | Cryptochrome-1 | <1e180 | 2,199 | |
| Accessory Proteins | |||||
| VRILLE (VRI) | |||||
| LpVRI | E0VHE1 | Pediculus humanus | Transcriptional factor nfil3/e4bp4, putative | 1e-43 | 424 |
| B7PEG8 | Ixodes scapularis | Transcriptional factor nfil3/e4bp4, putative | 9e-43 | 417 | |
| Q699T4 | Antheraea pernyi | Vrille | 6e-42 | 410 | |
| Q1XD36 | Danaus plexippus | Vrille | 1e-41 | 408 | |
| J9K7G7 | Acyrthosiphon pisum | Uncharacterized protein | 4e-40 | 403 | |
| CLOCK WORK ORANGE (CWO) | |||||
| LpCWO | B7PGM4 | Ixodes scapularis | Putative uncharacterized protein | 1e-57 | 528 |
| B4LW47 | Drosophila virilis | GJ23560 | 2e-42 | 430 | |
| Q7QFU3 | Anopheles gambiae | AGAP003844-PA | 2e-41 | 422 | |
| E9GEU4 | Daphnia pulex | Putative uncharacterized protein | 7e-41 | 395 | |
| B4JIN1 | Drosophila grimshawi | GH19128 | 2e-40 | 415 | |
| SUPERNUMERARY LIMBS1 (SLIMB1) | |||||
| LpSLIMB1 | L7M0T0 | Rhipicephalus pulchellus | Uncharacterized protein | <1e180 | 2,159 |
| E9IHL0 | Solenopsis invicta | Putative uncharacterized protein | <1e180 | 2,159 | |
| D2XMQ7 | Saccoglossus kowalevskii | Beta-TCRP E3 ligase | <1e180 | 2,145 | |
| H9HC45 | Atta cephalotes | Uncharacterized protein | <1e180 | 2,142 | |
| D6WA15 | Tribolium castaneum | Supernumerary limbs | <1e180 | 2,138 | |
| SUPERNUMERARY LIMBS2 (SLIMB2) | |||||
| LpSLIMB2 | L7M0T0 | Rhipicephalus pulchellus | Uncharacterized protein | <1e180 | 2,153 |
| E9HMX3 | Daphnia pulex | Putative uncharacterized protein | <1e180 | 2,150 | |
| E9IHL0 | Solenopsis invicta | Putative uncharacterized protein | <1e180 | 2,147 | |
| H3JK44 | Strongylocentrotus purpuratus | Uncharacterized protein | <1e180 | 2,135 | |
| G3I574 | Cricetulus griseus | F-box/WD repeat-containing protein 1A | <1e180 | 2,133 | |
| ARYL HYDROCARBON RECEPTOR NUCLEAR TRANSPORTER1 (ARNT1) | |||||
| LpARNT1 | C7B7E8 | Litopenaeus vannamei | Hypoxia inducible factor 1 beta | <1e180 | 1,799 |
| E0VXW6 | Pediculus humanus | Putative uncharacterized protein | <1e180 | 1,805 | |
| L7MH74 | Rhipicephalus pulchellus | Putative tango | <1e180 | 1,770 | |
| D6W6H5 | Tribolium castaneum | Putative uncharacterized protein | <1e180 | 1,739 | |
| Q1JUI4 | Daphnia magna | Aryl hydrocarbone receptor nuclear translocator | <1e180 | 1,723 | |
| ARYL HYDROCARBON RECEPTOR NUCLEAR TRANSPORTER2 (ARNT2) | |||||
| LpARNT2 | E0VXW6 | Pediculus humanus | Putative uncharacterized protein | <1e180 | 1,900 |
| C7B7E8 | Litopenaeus vannamei | Hypoxia inducible factor 1 beta | <1e180 | 1,858 | |
| Q1JUI4 | Daphnia magna | Aryl hydrocarbone receptor nuclear translocator | <1e180 | 1,850 | |
| Q1JUI5 | Daphnia magna | Aryl hydrocarbone receptor nuclear translocator | <1e180 | 1,838 | |
| E9FQM5 | Daphnia pulex | Putative aryl hydrocarbon receptor nuclear translocator | <1e180 | 1,838 | |
| CASIEN KINASE Iα (CKIα) | |||||
| LpCKIα | Q8JG73 | Danio rerio | Casein kinase 1alpha S | <1e180 | 1,570 |
| Q75WS8 | Carassius auratus | Casein kinase I alpha S | <1e180 | 1,570 | |
| Q8JGT0 | Danio rerio | Casein kinase 1, alpha 1 | <1e180 | 1,568 | |
| Q75WS9 | Carassius auratus | Casein kinase I alpha | <1e180 | 1,568 | |
| B5THN0 | Saccoglossus kowalevskii | Casein kinase 1 protein catalytic subunit | <1e180 | 1,567 | |
| CASIEN KINASE Iε (CKIε) | |||||
| LpXKIε | F6YKD5 | Equus caballus | Uncharacterized protein | <1e180 | 1,422 |
| G1SGL5 | Oryctolagus cuniculus | Uncharacterized protein | <1e180 | 1,418 | |
| G1U4F8 | Oryctolagus cuniculus | Uncharacterized protein | <1e180 | 1,418 | |
| M7AVG9 | Chelonia mydas | Casein kinase I isoform epsilon | <1e180 | 1,416 | |
| H0V5Y3 | Cavia porcellus | Uncharacterized protein | <1e180 | 1,416 | |
| CASIEN KINASE IIα (CKIIα) | |||||
| LpCKIIα | E2AW17 | Camponotus floridanus | Casein kinase II subunit alpha | <1e180 | 1,674 |
| F4WU82 | Acromyrmex echinatior | Casein kinase II subunit alpha | <1e180 | 1,666 | |
| B7PVH2 | Ixodes scapularis | Mitogen-activated protein kinase, putative | <1e180 | 1,671 | |
| E2BH98 | Harpegnathos saltator | Casein kinase II subunit alpha | <1e180 | 1,669 | |
| K7GIG0 | Pelodiscus sinensis) | Uncharacterized protein | <1e180 | 1,665 | |
| CASIEN KINASE IIβ (CKIIβ) | |||||
| LpCKIIβ | Q71U52 | Cyprinus carpio | CK2 beta subunit | 5e-144 | 1,057 |
| Q6DEU1 | Xenopus tropicalis | Casein kinase 2, beta polypeptide | 5e-144 | 1,057 | |
| Q1LXD2 | Danio rerio | Casein kinase 2 beta | 5e-144 | 1,057 | |
| M4AI87 | Xiphophorus maculatus | Uncharacterized protein | 5e-144 | 1,057 | |
| J3SBX9 | Crotalus adamanteus | Casein kinase II subunit beta | 5e-144 | 1,057 | |
| JETLAG (JET) | |||||
| LpJET | K1P9I2 | Crassostrea gigas | F-box only protein 37 | 1e-37 | 354 |
| C3Y7V1 | Branchiostoma floridae | Putative uncharacterized protein | 8e-37 | 356 | |
| C3ZZX8 | Branchiostoma floridae | Putative uncharacterized protein | 6e-35 | 342 | |
| R7VDT7 | Capitella teleta | Uncharacterized protein | 1e-32 | 326 | |
| A7SQL5 | Nematostella vectensis | Predicted protein | 1e-31 | 320 | |
| SHAGGY (SGG) | |||||
| LpSGG | E2C155 | Harpegnathos saltator | Protein kinase shaggy | <1e180 | 1,478 |
| E9H6Q6 | Daphnia pulex | Glycogen synthase kinase 3 beta | <1e180 | 1,475 | |
| E9I8X0 | Solenopsis invicta | Putative uncharacterized protein | <1e180 | 1,469 | |
| G3MII6 | Amblyomma maculatum | Putative uncharacterized protein | <1e180 | 1,453 | |
| F4X3V9 | Acromyrmex echinatior | Protein kinase shaggy | <1e180 | 1,467 | |
| PROTEIN PHOSPHOTASE 1α (PP1α) | |||||
| LpPP1α | K1PXG6 | Crassostrea gigas | Serine/threonine-protein phosphatase | <1e180 | 1,631 |
| A7RVJ0 | Nematostella vectensis | Serine/threonine-protein phosphatase | <1e180 | 1,627 | |
| E9HKA6 | Daphnia pulex | Serine/threonine-protein phosphatase | <1e180 | 1,624 | |
| H2L9G2 | Oryzias latipes | Serine/threonine-protein phosphatase | <1e180 | 1,620 | |
| H9KP59 | Apis mellifera | Serine/threonine-protein phosphatase | <1e180 | 1,618 | |
| PROTEIN PHOSPHOTASE 1β (PP1β) | |||||
| LpPP1β | L7MB61 | Rhipicephalus pulchellus | Serine/threonine-protein phosphatase | <1e180 | 1,470 |
| H2UK23 | Takifugu rubripes | Serine/threonine-protein phosphatase | <1e180 | 1,458 | |
| H3DIM0 | Tetraodon nigroviridis | Serine/threonine-protein phosphatase | <1e180 | 1,457 | |
| H2MDU5 | Oryzias latipes | Serine/threonine-protein phosphatase | <1e180 | 1,457 | |
| G3P7R9 | Gasterosteus aculeatus | Serine/threonine-protein phosphatase | <1e180 | 1,457 | |
| PROTEIN PHOSPHOTASE 2α-mts (PP2α-mts) | |||||
| LpPP2α-mts | L7M975 | Rhipicephalus pulchellus | Serine/threonine-protein phosphatase | <1e180 | 1,336 |
| B7QGY5 | Ixodes scapularis | Serine/threonine-protein phosphatase | <1e180 | 1,335 | |
| E0VKT2 | Pediculus humanus | Serine/threonine-protein phosphatase | <1e180 | 1,333 | |
| R4G8Q4 | Rhodnius prolixus | Putative serine/threonine protein phosphatase | <1e180 | 1,330 | |
| E9IAY8 | Solenopsis invicta | Serine/threonine-protein phosphatase | <1e180 | 1,334 | |
| PROTEIN PHOSPHOTASE 2α-WBT (PP2α-WBT) | |||||
| LpPP2α-WBT | L7M3M4 | Rhipicephalus pulchellus | Putative serine/threonine protein phosphatase… | <1e180 | 2,137 |
| H9J639 | Bombyx mori | Uncharacterized protein | <1e180 | 2,073 | |
| E2AI01 | Camponotus floridanus | Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit | <1e180 | 2,045 | |
| H9K2X9 | Apis mellifera | Uncharacterized protein | <1e180 | 2,044 | |
| E0VNG0 | Pediculus humanus | Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit | <1e180 | 2,041 | |
| PROTEIN PHOSPHOTASE 2α-TWS (PP2α-TWS) | |||||
| LpPP2α-TWS | H9KM79 | Apis mellifera | Uncharacterized protein | <1e180 | 2,081 |
| I3RJJ3 | Scylla paramamosain | Protein phosphatase 2A regulatory subunit B | <1e180 | 2,074 | |
| G3MLH6 | Amblyomma maculatum | Putative uncharacterized protein | <1e180 | 2,070 | |
| L7M886 | Rhipicephalus pulchellus | Putative serine/threonine protein phosphatase | <1e180 | 2,063 | |
| F4WRB8 | Acromyrmex echinatior | Protein phosphatase PP2A 55 kDa regulatory subunit | <1e180 | 2,055 | |
| Input Pathway Protein | |||||
| CRYPTOCHROME 1 (CRY1)† | |||||
| LpCRY1 | A2A264 | Dianemobius nigrofasciatus | Cryptochrome | 4e-132 | 1,022 |
| K1PRK6 | Crassostrea gigas | Cryptochrome-1 | 3e-120 | 941 | |
| R7V1J3 | Capitella teleta | Uncharacterized protein | 5e-119 | 937 | |
| Q2TJN5 | Danaus plexippus | Cryptochrome | 5e-115 | 908 | |
| J9T2S0 | Mythimna separata | Cryptochrome 1 | 4e-112 | 888 | |
| Output Pathway Proteins | |||||
| NEUROPEPTIDE F RECEPTOR (NPFR)† | |||||
| LpNPFR | T1KR44 | Tetranychus urticae | Uncharacterized protein | 6e-96 | 759 |
| T1IW42 | Strigamia maritima | Uncharacterized protein | 7e-88 | 707 | |
| B0WJ64 | Culex quinquefasciatus | G-protein coupled receptor | 3e78 | 642 | |
| Q16SC4 | Aedes aegypti | AAEL010626-PA | 5e-78 | 636 | |
| T1JTP7 | Tetranychus urticae | Uncharacterized protein | 1e-77 | 642 | |
| NEUROPEPTIDE F (NPF) | |||||
| LpNPF | T1KF83 | Tetranychus urticae | Uncharacterized protein | 23e-12 | 156 |
| A0A087UNM1 | Stegodyphus mimosarum | Uncharacterized protein | 160e-9 | 128 | |
| T1JM02 | Strigamia maritima | Uncharacterized protein | 670e-9 | 125 | |
| F6KM62 | Litopenaeus vannamei | Preproneuropeptide F I | 0.0000013 | 123 | |
| F6KM64 | Melicertus marginatus | Preproneuropeptide F I | 0.0000013 | 123 | |
| PIGMENT DISPERSING HORMONE RECEPTOR (PDHR)† | |||||
| LpPDHR | K7JRN8 | Nasonia vitripennis | Uncharacterized protein | 2.4e-63 | 548 |
| U3U904 | Nilaparvata lugens | Neuropeptide GPCR B2 | 95e-63 | 545 | |
| Q7PQE3 | Anopheles gambiae | AGAP003654-PA | 830e-63 | 537 | |
| A0A0P5AQT3 | Daphnia magna | Class b secretin g-protein coupled receptor | 590e-63 | 533 | |
| A0A0P5CML9 | Daphnia magna | Class b secretin g-protein coupled receptor | 1.4e-60 | 533 | |
†
indicates partial sequence.