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Flow Cytometry Engraftment Analysis. Gating strategy is shown for determination of common human T cell markers. Viable single human CD45+ cells are subgated into human CD3+ T cell subsets of interest, including CD4+ and CD8+ cells, as well as naïve (CD45RA+CCR7+), effector (CD45RA+CCR7−), central memory (CD45RA−CCR7+), and effector memory (CD45RA−CCR7−) CD8+ T cells.
