TABLE 2.

Performance of LAMP-ST131 with E. coli isolates, human urines, and stool swabs^a

	LAMP-ST131 positive	LAMP-ST131 negative
Well-characterized global E. coli collection (n = 260)
ST1c131 (n = 84)b	84	0
Non-ST131 (n = 176)c	0	176
Calgary clinical blood E. coli collection (n = 460)
ST131 PCR positive (n = 111)	111	0
ST131 PCR negative (n = 349)	6d	343
Urines (n = 550)
ST131 culture positive (n = 42)	41	1
ST131 culture negative (n = 508)	39	469
Stool swabs (n = 278)
ST131 culture positive (n = 30)	30	0
ST131 culture negative (n = 248)	40	208

^a LAMP-ST131 with well-characterized isolates showed a sensitivity of 100% (95% C.I. = 95.63–100%]) a specificity of 100% (95% C.I. = 97.86–100%); with clinical blood isolates, it showed a sensitivity of 100% (95% C.I. = 96.65–100%) and a specificity of 98.28% (95% C.I. = 96.30–99.21%); with urines, it showed a sensitivity of 97.6% (95% C.I. = 89.68–94.33%) and a specificity of 92.3% (95% C.I. = 87.68–99.88%); with stools, it showed a sensitivity of 100% (95% C.I. = 88.65–100) and a specificity of 83.87% (95% C.I. = 78.78–87.93%).

^b STc131 consisted of ST131 (n = 77) and ST131 SLVs (n = 7). The ST131 isolates belonged to the following clades: A (n = 10), B (n = 15), C0 (n = 5), C1-nonM27 (n = 20), C1-M27 (n = 4), and C2 (n = 23). The ST131 SLVs belonged to ST4171, ST5941, ST9126, ST12149, and ST12151 (one each) and two ST8671 isolates.

^c Non-ST131 isolates consisted of the following: ST101 (n = 1), ST10 (n = 3), ST12 (n = 6), ST69 (n = 4), ST11316 (n = 1), ST117 (n = 2), ST1193 (n = 5), ST1196 (n = 1), ST127 (n = 3), ST1312 (n = 1), ST14 (n = 10), ST141 (n = 3), ST1431 (n = 2), ST144 (n = 3), ST155 (n = 1), ST156 (n = 2), ST167 (n = 6), ST182 (n = 1), ST1858 (n = 1), ST1876 (n = 3), ST2003 (n = 2), ST205 (n = 1), ST2038 (n = 2), ST224 (n = 2), ST297 (n = 1), ST34 (n = 1), ST345 (n = 1), ST3478 (n = 1), ST349 (n = 1), ST354 (n = 2), ST357 (n = 1), ST361 (n = 3), ST372 (n = 2), ST38 (n = 6), ST393 (n = 3), ST399 (n = 1), ST4 (n = 1), ST404 (n = 8), ST405 (n = 4), ST409 (n = 4), ST410 (n = 6), ST4167 (n = 1), ST428 (n = 1), ST429 (n = 2), ST43 (n = 1), ST44 (n = 2), ST443 (n = 1), ST448 (n = 3), ST453 (n = 3), ST457 (n = 2), ST46 (n = 2), ST491 (n = 1), ST5021 (n = 1), ST538 (n = 1), ST540 (n = 1), ST550 (n = 3), ST568 (n = 4), ST569 (n = 4), ST58 (n = 1), ST582 (n = 1), ST617 (n = 2), ST624 (n = 1), ST648 (n = 4), ST69 (n = 3), ST73 (n = 5), ST88 (n = 3), and ST95 (n = 12).

^d The isolates belonged to ST1193 (n = 1), ST73 (n = 3), and ST95 (n = 2).