TABLE 2.
Efficacy of RaPID/BSI in removing human DNA from whole blood
| Leukocyte count (cells/mL) | Percent of normal leukocyte counta (%) | Source of sample | Human DNA removal yieldb (%) |
|---|---|---|---|
| ~6.7 × 106 | ~100 (baseline) |
Human whole blood | 99.98 ± 0.01 |
| ~1.5 × 107 | ~220 | Human whole blood spiked with isolated human leukocytes | 99.97 ± 0.01 |
| ~2.5 × 107 | ~370 | Human whole blood spiked with isolated human leukocytes | 99.94 ± 0.02 |
a
Normal leukocyte count in a healthy adult is 4.5–11.0 × 106 cells/mL (for baseline, the average leukocyte concentration of human whole blood was used in the experiments, namely 6.7 × 106 cells/mL).
b
Data are expressed as % of human DNA isolated using SLS/magnetic capture versus CTAB, mean ± SD from three to nine experiments.