TABLE 3.
Effect of select antimicrobial agents on the performance of RaPID/BSI
| Microorganism | Antimicrobial agent | Concentration | Identification of the target microorganism in the presence of the antimicrobial agent | ||
|---|---|---|---|---|---|
| Success rate % (N)a |
Normalized RaPID/BSI assay signal (mean ± SD) | ||||
| (−) Agent | (+) Agent | ||||
| S. aureus | Ampicillin | 152 µM | 100% (3/3) | 1.00 ± 0.18 | 0.98 ± 0.08 |
| Vancomycin | 69 µM | 100% (3/3) | 1.06 ± 0.24 | ||
| Gentamicin | 21 µM | 100% (3/3) | 1.02 ± 0.18 | ||
| Meropenem | 186 µM | 100% (3/3) | 1.22 ± 0.15 | ||
| Piperacillin and tazobactam | 117 and 19 µg/mL |
100% (3/3) | 0.94 ± 0.06 | ||
| E. coli | Ampicillin | 152 µM | 100% (3/3) | 1.00 ± 0.15 | 0.94 ± 0.14 |
| Vancomycin | 69 µM | 100% (3/3) | 1.14 ± 0.04 | ||
| Gentamicin | 21 µM | 100% (3/3) | 1.19 ± 0.15 | ||
| Meropenem | 186 µM | 100% (3/3) | 1.20 ± 0.04 | ||
| Piperacillin and tazobactam | 117 and 19 µg/mL |
100% (3/3) | 0.91 ± 0.13 | ||
| C. albicans | Ampicillin | 152 µM | 100% (3/3) | 1.00 ± 0.12 | 1.05 ± 0.16 |
| Vancomycin | 69 µM | 100% (3/3) | 1.03 ± 0.24 | ||
| Gentamicin | 21 µM | 100% (3/3) | 1.04 ± 0.14 | ||
| Meropenem | 186 µM | 100% (3/3) | 0.93 ± 0.02 | ||
| Piperacillin and tazobactam | 117 and 19 µg/mL |
100% (3/3) | 0.89 ± 0.10 | ||
a
Successful detection is defined as an obtained signal from the detection channel being above the established cutoff, determined as greater than the limit of blank (>3× SD the mean signal obtained in the same detection channel from a non-contrived sample).