TABLE 2.

FACS analysis of CV-1 cells^a

Protein	Transfection efficiency (%)b	Mean fluorescent index (a.u.)c	Relative mean fluorescence index (%)d
H/H/H	47	598.89	100.0
H/H/F	33	324.88	54.2
H/F/H	51	437.14	73.0
H/F/F	44	375.16	62.6
H/HF/F	29	302.32	50.5
H/H/N	30	210.97	35.2
H/H/R	30	129.82	21.7

^aThe histograms shown in Fig. 2 were analyzed to obtain the efficiency of the transfection of the CV-1 cells as well as the relative surface densities of the respective proteins in the expressing cells. 

^bTransfection efficiency was determined by calculating all cells which show fluorescence activity specific above that of the vac-control with respect to the overall number of measured cells (fluorescence intensities above 10² a.u. are determined to be specific). 

^cThe mean fluorescence index is a measure of the amount of protein per expressing cell in its membrane. Therefore, it is a useful parameter for the relative surface density of the expressed protein. 

^dThe relative mean fluorescence index was calculated with respect to H/H/H (wt HA). It shows that all chimeras were expressed at the surface of the CV-1 cells with a lower frequency than wt HA.