IC50 values and photocytotoxic indices (PI) for complex Pt-Fe in A2780 ovarian, A549 lung, PC3 prostate, T24 and SW-780 bladder cancer, and MRC-5 lung fibroblast cells after 1 h incubation, 1 h irradiation (blue 465 nm, green 520 nm) and 24 h recovery. CDDP (cisplatin) and complex 1 were studied for comparison.
| Cell | IC50a (μM) | ||||
|---|---|---|---|---|---|
| Pt–Fe | 1 (ref. 53) | CDDP | |||
| A2780 | Dark | >60 | >100 | >100 | |
| 465 nm (0.5 h) | 15.4 ± 1.2 | > 60 | n.d. | ||
| 465 nm | 1.5 ± 0.1 | 7.1 ± 0.4 | >100 | ||
| 520 nm | 3.8 ± 0.3 | >100 | n.d. | ||
| PI | Blue | >33 | >14 | — | |
| Green | >13 | — | — | ||
| A549 | Dark | >100 | >100 | >100 | |
| 465 nm | 26.9 ± 0.9 | 51.9 ± 2.5 | >100 | ||
| 520 nm | 41.3 ± 1.8 | >100 | n.d. | ||
| PI | Blue | >3.7 | >1.9 | — | |
| Green | >2.4 | — | — | ||
| PC3 | Dark | >100 | >100 | >100 | |
| 465 nm | 4.2 ± 0.4 | 55.6 ± 0.9 | >100 | ||
| 520 nm | 9.0 ± 0.1 | >100 | n.d. | ||
| PI | Blue | >24 | >1.7 | — | |
| Green | >11 | — | — | ||
| T24 | Dark | >100 | >100 | >100 | |
| 465 nm | 7.5 ± 0.4 | >100 | >100 | ||
| 520 nm | 32.8 ± 1.6 | n.d. | n.d. | ||
| PI | Blue | >13 | — | — | |
| Green | >3.0 | — | — | ||
| SW780 | Dark | >100 | >100 | >100 | |
| 465 nm | 25.9 ± 2.5 | >100 | >100 | ||
| 520 nm | 41.9 ± 0.9 | >100 | >100 | ||
| PI | Blue | >3.8 | — | — | |
| Green | >2.4 | — | — | ||
| MRC5 | Dark | >100 | >100 | >100 | |
a
Each value is mean of two independent experiments. n.d. = not determined.