Fig. 7. Rate constants for radical formation vs. pH using [Ru(bpy)3]3+ as oxidant for 2MP-α3C (orange dots), 2MP-α3C-E13A (grey squares), and 4MP-α3C (green triangles), compared to previously published data for α3Y (black circles).13 Standard deviations are shown, but are often smaller than the size of the data symbols. Samples contained 60–620 μM protein, 30 μM [Ru(bpy)3]2+, and 4–6 mM [Co(NH3)5Cl]2+. Fits according to eqn (1) are shown as lines (see Table 4 for results).
