. 2024 Jan 25;15(11):3957–3970. doi: 10.1039/d3sc05450k

α₃X midpoint reduction potentials, E°′, and pK_a values ± SD^a.

Protein	E°′(X₃₂˙/X₃₂)/mV		E°(X₃₂˙/X₃₂⁻) mV^b	pK_a
Protein	At pH 5.5	At pH 8.5	E°(X₃₂˙/X₃₂⁻) mV^b	pK_a
α₃Y	1065 (±2)	904 (±3)	749 (±4)	11.3 (±0.1)
2MP-α₃C	1011 (±3)	847 (±2)	780 (±4)	9.7 (±0.2)^c
4MP-α₃C	890 (±10)	715 (±10)	654 (±10)	9.5 (±0.1)
2MP-α₃C-E₁₃A	—	—		9.2 (±0.2)^c

Potentials (vs. NHE) and pK_a values were obtained from ref. 5.

Determined from α₃X Pourbaix diagrams⁵ at pH >> pK_a of reduced X₃₂.

Determined in the present work (Fig. S1 and S2).

α3X midpoint reduction potentials, E°′, and pKa values ± SDa.