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. Author manuscript; available in PMC: 2024 Mar 13.
Published in final edited form as: J Cell Physiol. 2021 Apr 30;236(11):7578–7590. doi: 10.1002/jcp.30401

FIGURE 2.

FIGURE 2

The ablation of TIGAR increases angiogenesis of MAEC. (a)–(d) The representative images of the tube-formation assay at 12 h of incubation and quantification of the number of master segments and the total length of the tube-like structures in the indicated groups (n = 4–6). **p < .01. Bar = 500 μm. (e) Cell proliferation of TIGAR KO MAEC was significantly higher than that of the WT MAECs, as measured by MTT assay (n = 10). ***p < .001. (f) The representative images of wound scratch assay at 0 and 12 h of incubation and quantification of MAEC migration in the indicated groups (n = 3–8). **p < .01. Bar = 250 μm. (g) The representative images of aortic ring sprouting assay at Day 5 of incubation and quantification of the sprouting area in the indicated groups (n = 6–12). Bar = 250 μm. ***p < .001. KO, knockout; MAECs, mouse aortic endothelial cells; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; TIGAR, TP53-induced glycolysis and apoptosis regulator; WT, wild type